Characterization of a stem cell population in lung cancer A549 cells

Sung, Ji-Min; Cho, Hee-Jung; Yi, Hee; Lee, Chi-Ho; Kim, Hye-Sun; Kim, Dong-Ku; Abd El-Aty, A M; Kim, Jin-Suk; Landowski, Christopher P; Hediger, Matthias A; Shin, Ho-Chul (2008). Characterization of a stem cell population in lung cancer A549 cells. Biochemical and biophysical research communications, 371(1), pp. 163-7. Orlando, Fla.: Academic Press 10.1016/j.bbrc.2008.04.038

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We isolated a stem cell subpopulation from human lung cancer A549 cells using FACS/Hoechst 33342. This side population (SP), which comprised 24% of the total cell population, totally disappeared after treatment with the selective ABCG 2 inhibitor fumitremorgin C. In a repopulation study, isolated SP and non-SP cells were each able to generate a heterogeneous population of SP and non-SP cells, but this repopulation occurred more rapidly in SP cells than non-SP. An MTT assay and cell cycle distribution analysis reveal a similar profile between SP and non-SP groups. However, in the presence of doxorubicin (DOX) and methotrexate (MTX), SP cells showed significantly lower Annexin V staining when compared to non-SP cells. Taken together, these results demonstrate that SP cells have an active regeneration capacity and high anti-apoptotic activity compared with non-SP cells. Furthermore, our GeneChip data revealed a heightened mRNA expression of ABCG2 and ABCC2 in SP cells. Overall these data explain why the SP of A549 has a unique ability to resist DOX and MTX treatments. Therefore, we suggest that the expression of the ABCG2 transporter plays an important role in the multidrug resistance phenotype of A549 SP cells.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
UniBE Contributor:	Hediger, Matthias
ISSN:	0006-291X
ISBN:	18423378
Publisher:	Academic Press
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:03
Last Modified:	05 Dec 2022 14:19
Publisher DOI:	10.1016/j.bbrc.2008.04.038
PubMed ID:	18423378
Web of Science ID:	000255923900033
URI:	https://boris.unibe.ch/id/eprint/27333 (FactScience: 106071)