Specific roles of Rac1 and Rac2 in motile functions of HT1080 fibrosarcoma cells

Niggli, Verena; Schlicht, Dominique; Affentranger, Sarah (2009). Specific roles of Rac1 and Rac2 in motile functions of HT1080 fibrosarcoma cells. Biochemical and biophysical research communications, 386(4), pp. 688-92. Orlando, Fla.: Academic Press 10.1016/j.bbrc.2009.06.098

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Rho family proteins are constitutively activated in the highly invasive human fibrosarcoma HT1080 cells. We now investigated the specific roles of Rac1 and Rac2 in regulating morphology, F-actin organization, adhesion, migration, and chemotaxis of HT1080 cells. Downregulation of Rac1 using specific siRNA probes resulted in cell rounding, markedly decreased spreading, adhesion, and chemotaxis of HT1080 cells. 2D migration on laminin-coated surfaces in contrast was not markedly affected. Selective Rac2 depletion did not affect cell morphology, cell adhesion, and 2D migration, but significantly reduced chemotaxis. Downregulation of both Rac1 and Rac2 resulted in an even more marked reduction, but not complete abolishment, of chemotaxis indicating distinct as well as overlapping roles of both proteins in chemotaxis. Rac1 thus is selectively required for HT1080 cell spreading and adhesion whereas Rac1 and Rac2 are both required for efficient chemotaxis.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology > Inflammatory Pathology

UniBE Contributor:

Niggli, Verena; Schlicht, Dominique Elisabeth and Affentranger, Sarah

ISSN:

0006-291X

ISBN:

19555660

Publisher:

Academic Press

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:08

Last Modified:

04 May 2014 23:21

Publisher DOI:

10.1016/j.bbrc.2009.06.098

PubMed ID:

19555660

Web of Science ID:

000268462100027

URI:

https://boris.unibe.ch/id/eprint/30006 (FactScience: 165662)

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