Specific roles of Rac1 and Rac2 in motile functions of HT1080 fibrosarcoma cells

Niggli, Verena; Schlicht, Dominique; Affentranger, Sarah (2009). Specific roles of Rac1 and Rac2 in motile functions of HT1080 fibrosarcoma cells. Biochemical and biophysical research communications, 386(4), pp. 688-92. Orlando, Fla.: Academic Press 10.1016/j.bbrc.2009.06.098

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Rho family proteins are constitutively activated in the highly invasive human fibrosarcoma HT1080 cells. We now investigated the specific roles of Rac1 and Rac2 in regulating morphology, F-actin organization, adhesion, migration, and chemotaxis of HT1080 cells. Downregulation of Rac1 using specific siRNA probes resulted in cell rounding, markedly decreased spreading, adhesion, and chemotaxis of HT1080 cells. 2D migration on laminin-coated surfaces in contrast was not markedly affected. Selective Rac2 depletion did not affect cell morphology, cell adhesion, and 2D migration, but significantly reduced chemotaxis. Downregulation of both Rac1 and Rac2 resulted in an even more marked reduction, but not complete abolishment, of chemotaxis indicating distinct as well as overlapping roles of both proteins in chemotaxis. Rac1 thus is selectively required for HT1080 cell spreading and adhesion whereas Rac1 and Rac2 are both required for efficient chemotaxis.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Service Sector > Institute of Pathology 04 Faculty of Medicine > Service Sector > Institute of Pathology > Inflammatory Pathology
UniBE Contributor:	Niggli, Verena, Schlicht, Dominique Elisabeth, Affentranger, Sarah
ISSN:	0006-291X
ISBN:	19555660
Publisher:	Academic Press
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:08
Last Modified:	05 Dec 2022 14:21
Publisher DOI:	10.1016/j.bbrc.2009.06.098
PubMed ID:	19555660
Web of Science ID:	000268462100027
URI:	https://boris.unibe.ch/id/eprint/30006 (FactScience: 165662)