Effects of endotoxin and catecholamines on hepatic mitochondrial respiration

Porta, Francesca; Bracht, Hendrik; Weikert, Christian; Beck, Mario; Takala, Jukka; Brandt, Sebastian; Hiltebrand, Luzius B; Jakob, Stephan M (2009). Effects of endotoxin and catecholamines on hepatic mitochondrial respiration. Inflammation, 32(5), pp. 315-21. New York, N.Y.: Springer US; http://www.springer-ny.com 10.1007/s10753-009-9138-y

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Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic of Intensive Care

UniBE Contributor:

Porta, Francesca Margherita; Bracht, Hendrik; Weikert, Christian; Beck, Mario; Takala, Jukka and Jakob, Stephan

ISSN:

0360-3997

Publisher:

Springer US; http://www.springer-ny.com

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:13

Last Modified:

23 Oct 2019 08:01

Publisher DOI:

10.1007/s10753-009-9138-y

PubMed ID:

19603262

Web of Science ID:

000270431800006

BORIS DOI:

10.7892/boris.32237

URI:

https://boris.unibe.ch/id/eprint/32237 (FactScience: 197247)

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