Porta, Francesca; Bracht, Hendrik; Weikert, Christian; Beck, Mario; Takala, Jukka; Brandt, Sebastian; Hiltebrand, Luzius B; Jakob, Stephan M (2009). Effects of endotoxin and catecholamines on hepatic mitochondrial respiration. Inflammation, 32(5), pp. 315-21. New York, N.Y.: Springer US; http://www.springer-ny.com 10.1007/s10753-009-9138-y
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Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic of Intensive Care |
UniBE Contributor: |
Porta, Francesca Margherita, Bracht, Hendrik, Weikert, Christian, Beck, Mario, Takala, Jukka, Jakob, Stephan |
ISSN: |
0360-3997 |
Publisher: |
Springer US; http://www.springer-ny.com |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 15:13 |
Last Modified: |
05 Dec 2022 14:22 |
Publisher DOI: |
10.1007/s10753-009-9138-y |
PubMed ID: |
19603262 |
Web of Science ID: |
000270431800006 |
BORIS DOI: |
10.7892/boris.32237 |
URI: |
https://boris.unibe.ch/id/eprint/32237 (FactScience: 197247) |
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