Probing the function of ionotropic and G protein-coupled receptors in surface-confined membranes

Danelon, Christophe; Terrettaz, Samuel; Guenat, Olivier; Koudelka, Milena; Vogel, Horst (2008). Probing the function of ionotropic and G protein-coupled receptors in surface-confined membranes. Methods, 46(2), pp. 104-15. San Diego, Calif.: Elsevier 10.1016/j.ymeth.2008.07.004

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This article reports on recent electrical and optical techniques for investigating cellular signaling reactions in artificial and native membranes immobilized on solid supports. The first part describes the formation of planar artificial lipid bilayers on gold electrodes, which reveal giga-ohm electrical resistance and the insertion and characterization of ionotropic receptors therein. These membranes are suited to record a few or even single ion channels by impedance spectroscopy. Such tethered membranes on planar arrays of microelectrodes offer mechanically robust, long-lasting measuring devices to probe the influence of different chemistries on biologically important ionotropic receptors and therefore will have a future impact to probe the function of channel proteins in basic science and in biosensor applications. In a second part, we present complementary approaches to form inside-out native membrane sheets that are immobilized on micrometer-sized beads or across submicrometer-sized holes machined in a planar support. Because the native membrane sheets are plasma membranes detached from live cells, these approaches offer a unique possibility to investigate cellular signaling processes, such as those mediated by ionotropic or G protein-coupled receptors, with original composition of lipids and proteins.

Item Type:

Journal Article (Original Article)

Division/Institute:

10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Lung Regeneration Technologies

UniBE Contributor:

Guenat, Olivier Thierry

ISSN:

1046-2023

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:24

Last Modified:

17 Mar 2015 22:52

Publisher DOI:

10.1016/j.ymeth.2008.07.004

PubMed ID:

18662784

Web of Science ID:

000261367600008

URI:

https://boris.unibe.ch/id/eprint/37888 (FactScience: 212174)

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