Potentiometric platform for the quantification of cellular potassium efflux

Generelli, Silvia; Jacquemart, Renaud; de Rooij, Nico F; Jolicoeur, Mario; Koudelka-Hep, Milena; Guenat, Olivier T (2008). Potentiometric platform for the quantification of cellular potassium efflux. Lab on a chip, 8(7), pp. 1210-5. Cambridge: Royal Society of Chemistry 10.1039/b801042k

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Renewed interest in the measurement of cellular K(+) effluxes has been prompted by the observation that potassium plays an active and important role in numerous key cellular events, in particular cell necrosis and apoptosis. Although necrosis and apoptosis follow different pathways, both induce intracellular potassium effluxes. Here, we report the use of potassium-selective microelectrodes located in a microfluidic platform for cell culture to monitor and quantify such effluxes in real time. Using this platform, we observed and measured the early signs of cell lysis induced by a modification of the extracellular osmolarity. Furthermore, we were able to quantify the number of dying cells by evaluating the extracellular potassium concentration. A comparison between the potentiometric measurement with a fluorescent live-dead assay performed under similar conditions revealed the delay between potassium effluxes and cell necrosis. These results suggest that such platforms may be exploited for applications, such as cytotoxicological screening assays or tumor cell proliferation assays, by using extracellular K(+) as cell death marker.

Item Type:

Journal Article (Original Article)

Division/Institute:

10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Lung Regeneration Technologies

UniBE Contributor:

Guenat, Olivier Thierry

ISSN:

1473-0197

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:24

Last Modified:

06 Dec 2013 14:06

Publisher DOI:

10.1039/b801042k

PubMed ID:

18584100

Web of Science ID:

000257236900031

URI:

https://boris.unibe.ch/id/eprint/37889 (FactScience: 212175)

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