Guenat, Olivier T; Generelli, Silvia; de Rooij, Nicolaas F; Koudelka-Hep, Milena; Berthiaume, François; Yarmush, Martin L (2006). Development of an array of ion-selective microelectrodes aimed for the monitoring of extracellular ionic activities. Analytical chemistry, 78(21), pp. 7453-60. Washington, D.C.: American Chemical Society 10.1021/ac0609733
Full text not available from this repository.In this study, we present the development and the characterization of a generic platform for cell culture able to monitor extracellular ionic activities (K+, NH4+) for real-time monitoring of cell-based responses, such as necrosis, apoptosis, or differentiation. The platform for cell culture is equipped with an array of 16 silicon nitride micropipet-based ion-selective microelectrodes with a diameter of either 2 or 6 microm. This array is located at the bottom of a 200-microm-wide and 350-microm-deep microwell where the cells are cultured. The characterization of the ion-selective microelectrode arrays in different standard and physiological solutions is presented. Near-Nernstian slopes were obtained for potassium- (58.6 +/- 0.8 mV/pK, n = 15) and ammonium-selective microelectrodes (59.4 +/- 3.9 mV/pNH4, n = 13). The calibration curves were highly reproducible and showed an average drift of 4.4 +/- 2.3 mV/h (n = 10). Long-term behavior and response after immersion in physiological solutions are also presented. The lifetime of the sensors was found to be extremely long with a high recovery rate.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Organs-on Chip Technologies |
UniBE Contributor: |
Guenat, Olivier Thierry |
ISSN: |
0003-2700 |
Publisher: |
American Chemical Society |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 15:24 |
Last Modified: |
05 Dec 2022 14:26 |
Publisher DOI: |
10.1021/ac0609733 |
PubMed ID: |
17073412 |
Web of Science ID: |
000241670000027 |
URI: |
https://boris.unibe.ch/id/eprint/37891 (FactScience: 212177) |
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