Development of an array of ion-selective microelectrodes aimed for the monitoring of extracellular ionic activities

Guenat, Olivier T; Generelli, Silvia; de Rooij, Nicolaas F; Koudelka-Hep, Milena; Berthiaume, François; Yarmush, Martin L (2006). Development of an array of ion-selective microelectrodes aimed for the monitoring of extracellular ionic activities. Analytical chemistry, 78(21), pp. 7453-60. Washington, D.C.: American Chemical Society 10.1021/ac0609733

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In this study, we present the development and the characterization of a generic platform for cell culture able to monitor extracellular ionic activities (K+, NH4+) for real-time monitoring of cell-based responses, such as necrosis, apoptosis, or differentiation. The platform for cell culture is equipped with an array of 16 silicon nitride micropipet-based ion-selective microelectrodes with a diameter of either 2 or 6 microm. This array is located at the bottom of a 200-microm-wide and 350-microm-deep microwell where the cells are cultured. The characterization of the ion-selective microelectrode arrays in different standard and physiological solutions is presented. Near-Nernstian slopes were obtained for potassium- (58.6 +/- 0.8 mV/pK, n = 15) and ammonium-selective microelectrodes (59.4 +/- 3.9 mV/pNH4, n = 13). The calibration curves were highly reproducible and showed an average drift of 4.4 +/- 2.3 mV/h (n = 10). Long-term behavior and response after immersion in physiological solutions are also presented. The lifetime of the sensors was found to be extremely long with a high recovery rate.

Item Type:

Journal Article (Original Article)

Division/Institute:

10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Lung Regeneration Technologies

UniBE Contributor:

Guenat, Olivier Thierry

ISSN:

0003-2700

Publisher:

American Chemical Society

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:24

Last Modified:

17 Mar 2015 22:52

Publisher DOI:

10.1021/ac0609733

PubMed ID:

17073412

Web of Science ID:

000241670000027

URI:

https://boris.unibe.ch/id/eprint/37891 (FactScience: 212177)

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