Nucleolar proteins regulate stage-specific gene expression and ribosomal RNA maturation in Trypanosoma brucei

Schumann Burkard, Gabriela; Käser, Sandro; de Araújo, Patricia; Schimanski, Bernd; Naguleswaran, Arunasalam; Knüsel, Sebastian; Heller, Manfred; Roditi, Isabel (2013). Nucleolar proteins regulate stage-specific gene expression and ribosomal RNA maturation in Trypanosoma brucei. Molecular microbiology, 88(4), pp. 827-840. Blackwell Science 10.1111/mmi.12227

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Different life-cycle stages of Trypanosoma brucei are characterized by stage-specific glycoprotein coats. GPEET procyclin, the major surface protein of early procyclic (insect midgut) forms, is transcribed in the nucleolus by RNA polymerase I as part of a polycistronic precursor that is processed to monocistronic mRNAs. In culture, when differentiation to late procyclic forms is triggered by removal of glycerol, the precursor is still transcribed, but accumulation of GPEET mRNA is prevented by a glycerol-responsive element in the 3' UTR. A genome-wide RNAi screen for persistent expression of GPEET in glycerol-free medium identified a novel protein, NRG1 (Nucleolar Regulator of GPEET 1), as a negative regulator. NRG1 associates with GPEET mRNA and with several nucleolar proteins. These include two PUF proteins, TbPUF7 and TbPUF10, and BOP1, a protein required for rRNA processing in other organisms. RNAi against each of these components prolonged or even increased GPEET expression in the absence of glycerol as well as causing a significant reduction in 5.8S rRNA and its immediate precursor. These results indicate that components of a complex used for rRNA maturation can have an additional role in regulating mRNAs that originate in the nucleolus.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Department of Clinical Research (DCR) > Unit Childrens Hospital > Protein- und Zellbiologie
04 Faculty of Medicine > Pre-clinic Human Medicine > Department of Clinical Research (DCR)
08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Schumann-Burkard, Gabriela; Käser, Sandro; Schimanski, Bernd; Naguleswaran, Arunasalam; Knüsel, Sebastian; Heller, Manfred and Roditi, Isabel

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

0950-382X

Publisher:

Blackwell Science

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Isabel Roditi

Date Deposited:

17 Mar 2014 12:29

Last Modified:

26 Sep 2017 22:30

Publisher DOI:

10.1111/mmi.12227

PubMed ID:

23617823

BORIS DOI:

10.7892/boris.45172

URI:

https://boris.unibe.ch/id/eprint/45172

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