A microfluidic platform for chemoresistive testing of multicellular pleural cancer spheroids

Ruppen, Janine; Cortes-Dericks, Lourdes; Marconi, Emanuele; Karoubi, Golnaz; Schmid, Ralph; Peng, Renwang; Marti, Thomas; Guenat, Olivier Thierry (2014). A microfluidic platform for chemoresistive testing of multicellular pleural cancer spheroids. Lab on a chip, 14(6), pp. 1198-1205. Royal Society of Chemistry 10.1039/c3lc51093j

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This study reports on a microfluidic platform on which single multicellular spheroids from malignant pleural mesothelioma (MPM), an aggressive tumor with poor prognosis, can be loaded, trapped and tested for chemotherapeutic drug response. A new method to detect the spheroid viability cultured on the microfluidic chip as a function of the drug concentration is presented. This approach is based on the evaluation of the caspase activity in the supernatant sampled from the chip and tested using a microplate reader. This simple and time-saving method does only require a minimum amount of manipulations and was established for very low numbers of cells. This feature is particularly important in view of personalised medicine applications for which the number of cells obtained from the patients is low. MPM spheroids were continuously perfused for 48 hours with cisplatin, one of the standard chemotherapeutic drugs used to treat MPM. The 50% growth inhibitory concentration of cisplatin in perfused MPM spheroids was found to be twice as high as in spheroids cultured under static conditions. This chemoresistance increase might be due to the continuous support of nutrients and oxygen to the perfused spheroids.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Thoraxchirurgie
10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Lung Regeneration Technologies

UniBE Contributor:

Ruppen, Janine; Cortes-Dericks, Lourdes; Marconi, Emanuele; Karoubi, Golnaz; Schmid, Ralph; Peng, Renwang; Marti, Thomas and Guenat, Olivier Thierry

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

1473-0197

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Colette Andrea Bichsel

Date Deposited:

18 Mar 2014 12:15

Last Modified:

26 Jan 2015 10:50

Publisher DOI:

10.1039/c3lc51093j

PubMed ID:

24496222

BORIS DOI:

10.7892/boris.46021

URI:

https://boris.unibe.ch/id/eprint/46021

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