Early over-expression of GRP receptors in prostatic carcinogenesis

Körner Jachertz, Meike; Waser, Beatrice; Rehmann, Ruth; Reubi, Jean-Claude (2014). Early over-expression of GRP receptors in prostatic carcinogenesis. Prostate, 74(2), pp. 217-224. Wiley-Blackwell 10.1002/pros.22743

[img] Text
pros22743.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (5MB) | Request a copy

BACKGROUND The GRP receptor shows high over-expression in prostatic adenocarcinoma and high grade PIN, but low expression in normal prostate glands. This represents the molecular basis for GRP receptor imaging of prostate cancer with radioactive compounds. However, a focal, high density GRP receptor expression can be observed in hitherto uncharacterized prostate glands. METHODS GRP receptors were quantitatively measured with in vitro receptor autoradiography using ¹²⁵I-Tyr⁴ -bombesin in samples from 115 prostates. On successive tissue sections, ¹²⁵I-Tyr⁴ -bombesin autoradiography was compared with H&E staining and MIB-1 and 34βE12 immunohistochemistry. RESULTS On one hand, it was confirmed that GRP receptors were expressed in adenocarcinoma and high grade PIN in high density and high incidence (77% and 73%, respectively), but in normal prostate glands in low density and low frequency (18%). On the other hand, a novel and intriguing observation was the existence of focal non-invasive prostate glands with high GRP receptor density, characterized by low grade nuclear atypia and increased proliferation, compatible with lower grade PIN. There was a significant GRP receptor density gradient (P ≤ 0.005), increasing from normal prostate glands (mean relative optical density, ROD, of ¹²⁵I-Tyr⁴ -bombesin binding: 0.17) over atypical glands without increased MIB-1 labeling (0.28) and atypical glands with increased MIB-1 expression (0.44) to high grade PIN and adenocarcinoma (0.64 and 0.58, respectively). CONCLUSIONS GRP receptor over-expression may be a novel, specific marker of early prostatic neoplastic transformation, arising in low grade PIN, and progressively increasing during malignant progression. This should be considered when interpreting in vivo GRP receptor imaging in males.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology
08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Körner Jachertz, Meike; Waser, Beatrice; Rehmann, Ruth and Reubi-Kattenbusch, Jean-Claude

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0270-4137

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Andrea Arnold

Date Deposited:

02 Apr 2014 14:47

Last Modified:

10 Oct 2019 14:30

Publisher DOI:

10.1002/pros.22743

PubMed ID:

24150752

Uncontrolled Keywords:

bombesin receptor, GRP receptor, prostate cancer, prostatic intraepithelial neoplasia, in vitro receptor autoradiography

BORIS DOI:

10.7892/boris.46327

URI:

https://boris.unibe.ch/id/eprint/46327

Actions (login required)

Edit item Edit item
Provide Feedback