Expression and function of psoriasin (S100A7) and koebnerisin (S100A15) in the brain

Jansen, Sandra; Podschun, Rainer; Leib, Stephen L.; Grötzinger, Joachim; Oestern, Stefanie; Michalek, Matthias; Pufe, Thomas; Brandenburg, Lars-Ove (2013). Expression and function of psoriasin (S100A7) and koebnerisin (S100A15) in the brain. Infection and immunity, 81(5), pp. 1788-1797. American Society for Microbiology 10.1128/IAI.01265-12

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The expression and function of psoriasin in the brain have been insufficiently characterized. Here, we show the induction of psoriasin expression in the central nervous system (CNS) after bacterial and viral stimulation. We used a pneumococcal meningitis in vivo model that revealed S100A15 expression in astrocytes and meningeal cells. These results were confirmed by a cell-based in vivo assay using primary rat glial and meningeal cell cultures. We investigated psoriasin expression in glial and meningeal cells using polyinosinic-polycytidylic acid, a synthetic analog of double-stranded RNA that mimics viral infection. Furthermore, previous results showed that antimicrobial peptides have not only bactericidal but also immunomodulatory functions. To test this statement, we used recombinant psoriasin as a stimulus. Glial and meningeal cells were treated with recombinant psoriasin at concentrations from 25 to 500 ng/ml. Treated microglia and meningeal cells showed phosphorylation of the extracellular signal-regulated kinase 1 (ERK1)/ERK2 (ERK1/2) signal transduction pathway. We demonstrated that this activation of ERK depends on RAGE, the receptor for advanced glycation end products. Furthermore, microglia cells treated with recombinant psoriasin change their phenotype to an enlarged shape. In conclusion, our results indicate an occurrence of psoriasin in the brain. An involvement of psoriasin as an antimicrobial protein that modulates the innate immune system after bacterial or viral stimulation is possible.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Leib, Stephen

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0019-9567

Publisher:

American Society for Microbiology

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Stephen Leib

Date Deposited:

26 May 2014 09:11

Last Modified:

05 Dec 2022 14:34

Publisher DOI:

10.1128/IAI.01265-12

PubMed ID:

23478321

BORIS DOI:

10.7892/boris.52656

URI:

https://boris.unibe.ch/id/eprint/52656

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