Apoptosis of hippocampal neurons in organotypic slice culture models: direct effect of bacteria revisited

Gianinazzi, Christian; Grandgirard, Denis; Simon, Franziska; Imboden, Hans; Joss, Philipp; Täuber, Martin G.; Leib, Stephen (2004). Apoptosis of hippocampal neurons in organotypic slice culture models: direct effect of bacteria revisited. Journal of neuropathology and experimental neurology, 63(6), pp. 610-617. Lippincott Williams & Wilkins

Text __nas-ifik.unibe.ch_ifik_Forschung_Gr_Leib_SHARED GROUP FOLDER_own papers_Apoptosis of Hippocampal Neurons in Organotypic Slice Culture Models Direct Effect of.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (382kB)

Neurons of the hippocampal dentate gyrus selectively undergo programmed cell death in patients suffering from bacterial meningitis and in experimental models of pneumococcal meningitis in infant rats. In the present study, a membrane-based organotypic slice culture system of rat hippocampus was used to test whether this selective vulnerability of neurons of the dentate gyrus could be reproduced in vitro. Apoptosis was assessed by nuclear morphology (condensed and fragmented nuclei), by immunochemistry for active caspase-3 and deltaC-APP, and by proteolytic caspase-3 activity. Co-incubation of the cultures with live pneumococci did not induce neuronal apoptosis unless cultures were kept in partially nutrient-deprived medium. Complete nutrient deprivation alone and staurosporine independently induced significant apoptosis, the latter in a dose-response way. In all experimental settings, apoptosis occurred preferentially in the dentate gyrus. Our data demonstrate that factors released by pneumococci per se failed to induce significant apoptosis in vitro. Thus, these factors appear to contribute to a multifactorial pathway, which ultimately leads to neuronal apoptosis in bacterial meningitis.

Interest & Impact

Downloads

1 since deposited on 01 Sep 2014
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item