Apoptosis of hippocampal neurons in organotypic slice culture models: direct effect of bacteria revisited

Gianinazzi, Christian; Grandgirard, Denis; Simon, Franziska; Imboden, Hans; Joss, Philipp; Täuber, Martin G.; Leib, Stephen (2004). Apoptosis of hippocampal neurons in organotypic slice culture models: direct effect of bacteria revisited. Journal of neuropathology and experimental neurology, 63(6), pp. 610-617. Lippincott Williams & Wilkins

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Neurons of the hippocampal dentate gyrus selectively undergo programmed cell death in patients suffering from bacterial meningitis and in experimental models of pneumococcal meningitis in infant rats. In the present study, a membrane-based organotypic slice culture system of rat hippocampus was used to test whether this selective vulnerability of neurons of the dentate gyrus could be reproduced in vitro. Apoptosis was assessed by nuclear morphology (condensed and fragmented nuclei), by immunochemistry for active caspase-3 and deltaC-APP, and by proteolytic caspase-3 activity. Co-incubation of the cultures with live pneumococci did not induce neuronal apoptosis unless cultures were kept in partially nutrient-deprived medium. Complete nutrient deprivation alone and staurosporine independently induced significant apoptosis, the latter in a dose-response way. In all experimental settings, apoptosis occurred preferentially in the dentate gyrus. Our data demonstrate that factors released by pneumococci per se failed to induce significant apoptosis in vitro. Thus, these factors appear to contribute to a multifactorial pathway, which ultimately leads to neuronal apoptosis in bacterial meningitis.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
08 Faculty of Science > Department of Biology > Institute of Cell Biology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Gianinazzi, Christian; Grandgirard, Denis; Simon, Franziska; Imboden, Hans; Joss, Philipp; Täuber, Martin G. and Leib, Stephen

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0022-3069

Publisher:

Lippincott Williams & Wilkins

Language:

English

Submitter:

Stephen Leib

Date Deposited:

01 Sep 2014 09:33

Last Modified:

08 Jul 2015 07:54

PubMed ID:

15217089

Web of Science ID:

000221897100005

BORIS DOI:

10.7892/boris.52736

URI:

https://boris.unibe.ch/id/eprint/52736 (FactScience: 60802)

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