Functional differentiation of spider hemocytes by light and transmission electron microscopy, and MALDI-MS-imaging

Kuhn-Nentwig, Lucia Gerda; Kopp, Lukas S.; Nentwig, Wolfgang; Haenni, Beat; Streitberger, Kathrin; Schürch, Stefan; Schaller, Johann (2014). Functional differentiation of spider hemocytes by light and transmission electron microscopy, and MALDI-MS-imaging. Developmental and Comparative Immunology, 43(1), pp. 59-67. Elsevier 10.1016/j.dci.2013.10.008

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The most abundant cell types in the hemolymph of Cupiennius salei are plasmatocytes (70–80%) and granulocytes (20–30%). Both cells differ in shape, cytochemical and transmission electron microscopy staining of their cytoplasma and granules. According to MALDI-IMS (matrix-assisted laser desorption ionization mass spectrometry imaging), granulocytes exhibit ctenidin 1 (9510 Da) and ctenidin 3 (9568 Da), SIBD-1 (8675 Da), and unknown peptides with masses of 2207 and 6239 Da. Plasmatocytes exhibit mainly a mass of 6908 Da. Unknown peptides with masses of 1546 and 1960 Da were detected in plasmatocytes and granulocytes. Transmission electron microscopy confirms the presence of two compounds in one granule and cytochemical staining (light microscopy) tends to support this view. Two further hemocyte types (cyanocytes containing hemocyanin and prehemocytes as stem cells) are only rarely detected in the hemolymph. These four hemocyte types constitute the cellular part of the spider immune system and this is discussed in view of arachnid hemocyte evolution.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Community Ecology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
08 Faculty of Science > Departement of Chemistry and Biochemistry
08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE)

UniBE Contributor:

Kuhn-Nentwig, Lucia Gerda; Nentwig, Wolfgang; Haenni, Beat; Schürch, Stefan and Schaller, Johann

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)
500 Science > 580 Plants (Botany)
600 Technology > 610 Medicine & health
500 Science > 540 Chemistry

ISSN:

0145-305X

Publisher:

Elsevier

Language:

English

Submitter:

Stefan Schürch

Date Deposited:

09 Feb 2015 09:55

Last Modified:

26 Aug 2015 15:57

Publisher DOI:

10.1016/j.dci.2013.10.008

BORIS DOI:

10.7892/boris.61860

URI:

https://boris.unibe.ch/id/eprint/61860

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