Cyclooxygenase-2 and 5-lipoxygenase in dogs with chronic enteropathies.

Dumusc, S D; Ontsouka, Edgar; Schnyder, M; Hartnack, S; Albrecht, C; Bruckmaier, Rupert; Burgener, I A (2014). Cyclooxygenase-2 and 5-lipoxygenase in dogs with chronic enteropathies. Journal of veterinary internal medicine, 28(6), pp. 1684-1691. Wiley-Blackwell 10.1111/jvim.12463

[img] Text
jvim12463.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (243kB) | Request a copy

BACKGROUND Cyclooxygenase-2 (COX-2) is a key enzyme in the synthesis of pro-inflammatory prostaglandins and 5-lipoxygenase (5-LO) is the major source of leukotrienes. Their role in IBD has been demonstrated in humans and animal models, but not in dogs with chronic enteropathies (CCE). HYPOTHESIS COX-2 and 5-LO are upregulated in dogs with CCE. ANIMALS Fifteen healthy control dogs (HCD), 10 dogs with inflammatory bowel disease (IBD), and 15 dogs with food-responsive diarrhea (FRD). METHODS Prospective study. mRNA expression of COX-2, 5-LO, IL-1b, IL-4, IL-6, TNF, IL-10 and TFG-β was evaluated by quantitative real-time RT-PCR in duodenal and colonic biopsies before and after treatment. RESULTS COX-2 expression in the colon was significantly higher in IBD and FRD before and after treatment (all P < .01). IL-1b was higher in FRD in the duodenum after treatment (P = .021). TGF-β expression was significantly higher in the duodenum of HCD compared to FRD/IBD before treatment (both P < .001) and IBD after treatment (P = .012). There were no significant differences among groups and within groups before and after treatment for IL-4, IL-6, TNF, and IL-10. There was a significant correlation between COX-2 and IL-1b in duodenum and colon before treatment in FRD and IBD, whereas 5-LO correlated better with IL-6 and TNF. IL-10 and TGF-β usually were correlated. CONCLUSIONS AND CLINICAL IMPORTANCE COX-2 is upregulated in IBD and FRD, whereas IL-1b and TGF-β seem to be important pro- and anti-inflammatory cytokines, respectively. The use of dual COX/5-LO inhibitors could be an interesting alternative in the treatment of CCE.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Physiology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Ontsouka, Edgar and Bruckmaier, Rupert

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0891-6640

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Barbara Järmann-Bangerter

Date Deposited:

12 Mar 2015 09:34

Last Modified:

02 Sep 2015 10:20

Publisher DOI:

10.1111/jvim.12463

PubMed ID:

25269796

Uncontrolled Keywords:

Food-responsive diarrhea, Inflammatory bowel disease, Interleukin, Leukotriene

BORIS DOI:

10.7892/boris.64359

URI:

https://boris.unibe.ch/id/eprint/64359

Actions (login required)

Edit item Edit item
Provide Feedback