PSGL-1 and E/P-selectins are essential for T-cell rolling in inflamed CNS microvessels but dispensable for initiation of EAE

Sathiyanadan, Karthik; Coisne, Caroline Marie; Enzmann, Gaby; Deutsch, Urban; Engelhardt, Britta (2014). PSGL-1 and E/P-selectins are essential for T-cell rolling in inflamed CNS microvessels but dispensable for initiation of EAE. European journal of immunology, 44(8), pp. 2287-2294. Wiley-VCH 10.1002/eji.201344214

[img] Text
eji2997.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (681kB) | Request a copy

T-cell migration across the blood-brain barrier is a crucial step in the pathogenesis of EAE, an animal model for MS. Live cell imaging studies demonstrated that P-selectin glycoprotein ligand-1 (PSGL-1) and its endothelial ligands E- and P-selectin mediate the initial rolling of T cells in brain vessels during EAE. As functional absence of PSGL-1 or E/P-selectins does not result in ameliorated EAE, we speculated that T-cell entry into the spinal cord is independent of PSGL-1 and E/P-selectin. Performing intravital microscopy, we observed the interaction of WT or PSGL-1(-/-) proteolipid protein-specific T cells in inflamed spinal cord microvessels of WT or E/P-selectin(-/-) SJL/J mice during EAE. T-cell rolling but not T-cell capture was completely abrogated in the absence of either PSGL-1 or E- and P-selectin, resulting in a significantly reduced number of T cells able to firmly adhere in the inflamed spinal cord microvessels, but did not lead to reduced T-cell invasion into the CNS parenchyma. Thus, PSGL-1 interaction with E/P-selectin is essential for T-cell rolling in inflamed spinal cord microvessels during EAE. Taken together with previous observations, our findings show that T-cell rolling is not required for successful T-cell entry into the CNS and initiation of EAE.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Sathiyanadan, Karthik; Coisne, Caroline Marie; Enzmann, Gaby; Deutsch, Urban and Engelhardt, Britta

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0014-2980

Publisher:

Wiley-VCH

Language:

English

Submitter:

Ursula Zingg-Zünd

Date Deposited:

07 Apr 2015 12:46

Last Modified:

24 Apr 2015 09:07

Publisher DOI:

10.1002/eji.201344214

PubMed ID:

24740164

Uncontrolled Keywords:

Blood-brain barrier Experimental autoimmune encephalomyelitis P-selectin P-selectin glycoprotein ligand-1 T-cell rolling

BORIS DOI:

10.7892/boris.66353

URI:

https://boris.unibe.ch/id/eprint/66353

Actions (login required)

Edit item Edit item
Provide Feedback