Separation of small metabolites and lipids in spectra from biopsies by diffusion-weighted HR-MAS NMR: a feasibility study.

Diserens, Gaëlle; Vermathen, Martina; Precht, Maria Christina; Broskey, N T; Boesch, Chris; Amati, F; Dufour, Jean-François; Vermathen, Peter (2015). Separation of small metabolites and lipids in spectra from biopsies by diffusion-weighted HR-MAS NMR: a feasibility study. The Analyst, 140(1), pp. 272-279. Royal Society of Chemistry 10.1039/c4an01663g

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High Resolution Magic Angle Spinning (HR-MAS) NMR allows metabolic characterization of biopsies. HR-MAS spectra from tissues of most organs show strong lipid contributions that are overlapping metabolite regions, which hamper metabolite estimation. Metabolite quantification and analysis would benefit from a separation of lipids and small metabolites. Generally, a relaxation filter is used to reduce lipid contributions. However, the strong relaxation filter required to eliminate most of the lipids also reduces the signals for small metabolites. The aim of our study was therefore to investigate different diffusion editing techniques in order to employ diffusion differences for separating lipid and small metabolite contributions in the spectra from different organs for unbiased metabonomic analysis. Thus, 1D and 2D diffusion measurements were performed, and pure lipid spectra that were obtained at strong diffusion weighting (DW) were subtracted from those obtained at low DW, which include both small metabolites and lipids. This subtraction yielded almost lipid free small metabolite spectra from muscle tissue. Further improved separation was obtained by combining a 1D diffusion sequence with a T2-filter, with the subtraction method eliminating residual lipids from the spectra. Similar results obtained for biopsies of different organs suggest that this method is applicable in various tissue types. The elimination of lipids from HR-MAS spectra and the resulting less biased assessment of small metabolites have potential to remove ambiguities in the interpretation of metabonomic results. This is demonstrated in a reproducibility study on biopsies from human muscle.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Clinical Radiology
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
08 Faculty of Science > Departement of Chemistry and Biochemistry
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Hepatology
04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology > DCR Magnetic Resonance Spectroscopy and Methodology (AMSM)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Diserens, Gaëlle; Vermathen, Martina; Precht, Maria Christina; Boesch, Christoph Hans; Dufour, Jean-François and Vermathen, Peter

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

1364-5528

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Christoph Hans Boesch

Date Deposited:

09 Apr 2015 09:28

Last Modified:

19 Apr 2016 11:47

Publisher DOI:

10.1039/c4an01663g

PubMed ID:

25368873

BORIS DOI:

10.7892/boris.66682

URI:

https://boris.unibe.ch/id/eprint/66682

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