A glycosylation mutant of Trypanosoma brucei links social motility defects in vitro to impaired colonisation of tsetse in vivo

Imhof, Simon; Vu Nguyen, Xuan Lan; Bütikofer, Peter; Roditi, Isabel (2015). A glycosylation mutant of Trypanosoma brucei links social motility defects in vitro to impaired colonisation of tsetse in vivo. Eukaryotic cell, 14(6), pp. 588-592. American Society for Microbiology ASM 10.1128/EC.00023-15

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Transmission of African trypanosomes by tsetse flies requires that the parasites migrate out of the midgut lumen and colonise the ectoperitrophic space. Early procyclic culture forms correspond to trypanosomes in the lumen; on agarose plates they exhibit social motility, migrating en masse as radial projections from an inoculation site. We show that an Rft1-/- mutant needs to reach a greater threshold number before migration begins, and that it forms fewer projections than its wild-type parent. The mutant is also up to 4 times less efficient at establishing midgut infections. Ectopic expression of Rft1 rescues social motility defects and restores the ability to colonise the fly. These results are consistent with social motility reflecting movement to the ectoperitrophic space, implicate N-glycans in the signalling cascades for migration in vivo and in vitro, and provide the first evidence that parasite-parasite interactions determine the success of transmission by the insect host.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Imhof, Simon; Vu Nguyen, Xuan Lan; Bütikofer, Peter and Roditi, Isabel

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1535-9778

Publisher:

American Society for Microbiology ASM

Funders:

[UNSPECIFIED] Howard Hughes Medical Institute
[4] Swiss National Science Foundation
[UNSPECIFIED] Canton of Bern

Language:

English

Submitter:

Isabel Roditi

Date Deposited:

08 May 2015 12:11

Last Modified:

26 Jun 2016 02:04

Publisher DOI:

10.1128/EC.00023-15

PubMed ID:

25862152

BORIS DOI:

10.7892/boris.68788

URI:

https://boris.unibe.ch/id/eprint/68788

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