Expression of the vault RNA protects cells from undergoing apoptosis

Amort, Melanie; Nachbauer, Birgit; Tuzlak, Selma; Kieser, Arnd; Schepers, Aloys; Villunger, Andreas; Polacek, Norbert (2015). Expression of the vault RNA protects cells from undergoing apoptosis. Nature communications, 6(7030), p. 7030. Nature Publishing Group 10.1038/ncomms8030

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Non-protein-coding RNAs are a functionally versatile class of transcripts exerting their biological roles on the RNA level. Recently, we demonstrated that the vault complex-associated RNAs (vtRNAs) are significantly upregulated in Epstein-Barr virus (EBV)-infected human B cells. Very little is known about the function(s) of the vtRNAs or the vault complex. Here, we individually express latent EBV-encoded proteins in B cells and identify the latent membrane protein 1 (LMP1) as trigger for vtRNA upregulation. Ectopic expression of vtRNA1-1, but not of the other vtRNA paralogues, results in an improved viral establishment and reduced apoptosis, a function located in the central domain of vtRNA1-1. Knockdown of the major vault protein has no effect on these phenotypes revealing that vtRNA1-1 and not the vault complex contributes to general cell death resistance. This study describes a NF-κB-mediated role of the non-coding vtRNA1-1 in inhibiting both the extrinsic and intrinsic apoptotic pathways.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Nachbauer, Birgit and Polacek, Norbert

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

2041-1723

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

21 May 2015 13:43

Last Modified:

12 Sep 2017 07:51

Publisher DOI:

10.1038/ncomms8030

BORIS DOI:

10.7892/boris.68902

URI:

https://boris.unibe.ch/id/eprint/68902

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