Felder, Marcel; Sallin, Pauline; Barbe, Laurent; Haenni, Beat; Gazdhar, Amiq; Geiser, Thomas; Guenat, Olivier (2012). Microfluidic wound-healing assay to assess the regenerative effect of HGF on wounded alveolar epithelium. Lab on a chip, 12(3), pp. 640-6. Cambridge: Royal Society of Chemistry 10.1039/c1lc20879a
Full text not available from this repository.We present a microfluidic epithelial wound-healing assay that allows characterization of the effect of hepatocyte growth factor (HGF) on the regeneration of alveolar epithelium using a flow-focusing technique to create a regular wound in the epithelial monolayer. The phenotype of the epithelial cell was characterized using immunostaining for tight junction (TJ) proteins and transmission electron micrographs (TEMs) of cells cultured in the microfluidic system, a technique that is reported here for the first time. We demonstrate that alveolar epithelial cells cultured in a microfluidic environment preserve their phenotype before and after wounding. In addition, we report a wound-healing benefit induced by addition of HGF to the cell culture medium (19.2 vs. 13.5 μm h(-1) healing rate).
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB [discontinued] 10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Organs-on Chip Technologies 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology |
UniBE Contributor: |
Felder, Marcel Christian, Sallin, Pauline, Haenni, Beat, Gazdhar, Amiq, Geiser, Thomas (A), Guenat, Olivier Thierry |
ISSN: |
1473-0197 |
Publisher: |
Royal Society of Chemistry |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:21 |
Last Modified: |
29 Mar 2023 23:32 |
Publisher DOI: |
10.1039/c1lc20879a |
PubMed ID: |
22146948 |
Web of Science ID: |
000298964300027 |
URI: |
https://boris.unibe.ch/id/eprint/7032 (FactScience: 212173) |
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