Microfluidic wound-healing assay to assess the regenerative effect of HGF on wounded alveolar epithelium

Felder, Marcel; Sallin, Pauline; Barbe, Laurent; Haenni, Beat; Gazdhar, Amiq; Geiser, Thomas; Guenat, Olivier (2012). Microfluidic wound-healing assay to assess the regenerative effect of HGF on wounded alveolar epithelium. Lab on a chip, 12(3), pp. 640-6. Cambridge: Royal Society of Chemistry 10.1039/c1lc20879a

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We present a microfluidic epithelial wound-healing assay that allows characterization of the effect of hepatocyte growth factor (HGF) on the regeneration of alveolar epithelium using a flow-focusing technique to create a regular wound in the epithelial monolayer. The phenotype of the epithelial cell was characterized using immunostaining for tight junction (TJ) proteins and transmission electron micrographs (TEMs) of cells cultured in the microfluidic system, a technique that is reported here for the first time. We demonstrate that alveolar epithelial cells cultured in a microfluidic environment preserve their phenotype before and after wounding. In addition, we report a wound-healing benefit induced by addition of HGF to the cell culture medium (19.2 vs. 13.5 μm h(-1) healing rate).

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB
10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Lung Regeneration Technologies
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology

UniBE Contributor:

Felder, Marcel; Sallin, Pauline; Haenni, Beat; Gazdhar, Amiq Ur Rahman; Geiser, Thomas and Guenat, Olivier Thierry

ISSN:

1473-0197

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:21

Last Modified:

06 Dec 2013 13:27

Publisher DOI:

10.1039/c1lc20879a

PubMed ID:

22146948

Web of Science ID:

000298964300027

URI:

https://boris.unibe.ch/id/eprint/7032 (FactScience: 212173)

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