Escherichia coli are susceptible to thiazolides if the tolc efflux system is inhibited

Müller, Joachim; Vaithilingam, Jathana; Rosner, Judah Lee; Müller, Norbert (2014). Escherichia coli are susceptible to thiazolides if the tolc efflux system is inhibited. Journal of Developing Drugs, 3(2), p. 1000124. OMICS Group 10.4172/2329-6631.1000124

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Nitazoxanide (NTZ) and other thiazolides are effective against intracellular protozoa’s, anaerobic or micro aerophilic bacteria, viruses and tumour cells. Concerning their potential effects against Escherichia coli, the published results are scarce and conflicting. In order to investigate whether thiazolides are effective against aerobically growing E. coli, we examined mutants of the TolC efflux system for their sensitivity to nitro thiazolides, including NTZ, and bromothiazolides. We determined the susceptibilities of tolC mutants to various thiazolides and found that tolC mutants of E. coli were susceptible to both nitro thiazolides and bromothiazolides indicating a mechanism of action different from nitro reduction. Moreover, we showed that thiazolides induced a spy:lacZ transcriptional fusion indicating that thiazolides generate stress in the bacterial envelope. Moreover, wild type strains became susceptible to thiazolides if the tolC efflux system was inhibited. Taken together, our results show that thiazolides are effective against E. coli if their export from the cells is impaired.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Müller, Joachim; Vaithilingam, Jathana and Müller, Norbert

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)

ISSN:

2329-6631

Publisher:

OMICS Group

Language:

English

Submitter:

Norbert Müller

Date Deposited:

14 Aug 2015 11:47

Last Modified:

14 Sep 2020 14:27

Publisher DOI:

10.4172/2329-6631.1000124

BORIS DOI:

10.7892/boris.71003

URI:

https://boris.unibe.ch/id/eprint/71003

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