Generation of Aptamers with an Expanded Chemical Repertoire

Diafa, Stella; Hollenstein, Marcel (2015). Generation of Aptamers with an Expanded Chemical Repertoire. Molecules, 20(9), pp. 16643-16671. Molecular Diversity Preservation International MDPI 10.3390/molecules200916643

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The enzymatic co-polymerization of modified nucleoside triphosphates (dN*TPs and N*TPs) is a versatile method for the expansion and exploration of expanded chemical space in SELEX and related combinatorial methods of in vitro selection. This strategy can be exploited to generate aptamers with improved or hitherto unknown properties. In this review, we discuss the nature of the functionalities appended to nucleoside triphosphates and their impact on selection experiments. The properties of the resulting modified aptamers will be described, particularly those integrated in the fields of biomolecular diagnostics, therapeutics, and in the expansion of genetic systems (XNAs).

Item Type:

Journal Article (Review Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Diafa, Stella and Hollenstein, Marcel

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

1420-3049

Publisher:

Molecular Diversity Preservation International MDPI

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Marcel Hollenstein

Date Deposited:

03 Nov 2015 14:22

Last Modified:

04 Nov 2015 10:47

Publisher DOI:

10.3390/molecules200916643

PubMed ID:

26389865

BORIS DOI:

10.7892/boris.72412

URI:

https://boris.unibe.ch/id/eprint/72412

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