Changes in mitochondrial enzymatic activities of monocytes during prolonged hypobaric hypoxia and influence of antioxidants: A randomized controlled study

Merz, Tobias Michael; Pichler Hefti, Jacqueline Renée; Hefti, Urs; Huber, Andreas; Jakob, Stephan; Takala, Jukka; Djafarzadeh, Siamak (2015). Changes in mitochondrial enzymatic activities of monocytes during prolonged hypobaric hypoxia and influence of antioxidants: A randomized controlled study. Redox report, 20(5), pp. 234-240. Maney Publishing 10.1179/1351000215Y.0000000007

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OBJECTIVES Exposure to high altitudes is associated with oxidative cellular damage due to the increased level of reactive oxygen and nitrogen species and altered activity of antioxidant systems. Subjects were submitted to prolonged hypoxia, to evaluate changes in mitochondrial enzyme activities of monocytes and their attenuation by supplementation with antioxidants. METHODS Twelve subjects were randomly assigned to receive antioxidant supplements or placebo prior to and during an expedition to Pik Lenin (7145 m). Monocytes were isolated from blood samples to determine the activity of mitochondrial enzymes cytochrome c oxidase and citrate synthase at 490 m (baseline) and at the altitudes of 3550 m, 4590 m, and 5530 m. RESULTS An increase in citrate synthase activity at all altitudes levels was observed. Hypoxia induced an increase in the activity of cytochrome c oxidase only at 4590 m. Neither citrate synthase activity nor cytochrome c oxidase activity differed between the subjects receiving antioxidant supplements and those receiving placebo. CONCLUSIONS Hypoxia leads to an increase in citrate synthase activity of monocyte mitochondria as a marker of mitochondrial mass, which is not modified by antioxidant supplementation. The increase in mitochondrial mass may represent a compensatory mechanism to preserve oxidative phosphorylation of monocytes at high altitudes.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic of Intensive Care
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pavillon 52 > Forschungsgruppe Intensivmedizin

UniBE Contributor:

Merz, Tobias Michael; Pichler Hefti, Jacqueline Renée; Jakob, Stephan; Takala, Jukka and Djafarzadeh, Siamak

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1743-2928

Publisher:

Maney Publishing

Language:

English

Submitter:

Alessandra Angelini

Date Deposited:

28 Jan 2016 16:25

Last Modified:

29 Feb 2016 15:19

Publisher DOI:

10.1179/1351000215Y.0000000007

PubMed ID:

25867847

Uncontrolled Keywords:

Antioxidants, Mitochondrial function, Mitochondrial mass, Oxidative stress, Peripheral blood monocytes

URI:

https://boris.unibe.ch/id/eprint/74854

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