Flagellar membranes are rich in raft-forming phospholipids.

Serricchio, Mauro; Schmid, Adrien W; Steinmann, Michael Eduard; Sigel, Erwin; Rauch, Monika; Julkowska, Daria; Bonnefoy, Serge; Fort, Cécile; Bastin, Philippe; Bütikofer, Peter (2015). Flagellar membranes are rich in raft-forming phospholipids. Biology open BiO, 4(9), pp. 1143-1153. Company of Biologists 10.1242/bio.011957

[img]
Preview
Text
Bütikofer Flagellar membranes.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (1MB) | Preview

The observation that the membranes of flagella are enriched in sterols and sphingolipids has led to the hypothesis that flagella might be enriched in raft-forming lipids. However, a detailed lipidomic analysis of flagellar membranes is not available. Novel protocols to detach and isolate intact flagella from Trypanosoma brucei procyclic forms in combination with reverse-phase liquid chromatography high-resolution tandem mass spectrometry allowed us to determine the phospholipid composition of flagellar membranes relative to whole cells. Our analyses revealed that phosphatidylethanolamine, phosphatidylserine, ceramide and the sphingolipids inositol phosphorylceramide and sphingomyelin are enriched in flagella relative to whole cells. In contrast, phosphatidylcholine and phosphatidylinositol are strongly depleted in flagella. Within individual glycerophospholipid classes, we observed a preference for ether-type over diacyl-type molecular species in membranes of flagella. Our study provides direct evidence for a preferential presence of raft-forming phospholipids in flagellar membranes of T. brucei.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Serricchio, Mauro; Steinmann, Michael Eduard; Sigel, Erwin; Rauch, Monika and Bütikofer, Peter

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2046-6390

Publisher:

Company of Biologists

Language:

English

Submitter:

Barbara Järmann-Bangerter

Date Deposited:

04 Mar 2016 08:10

Last Modified:

04 Mar 2016 08:10

Publisher DOI:

10.1242/bio.011957

PubMed ID:

26276100

Uncontrolled Keywords:

Flagella; Lipid rafts; Mass spectrometry; Membrane lipids; Sphingolipids; Trypanosoma brucei

BORIS DOI:

10.7892/boris.76344

URI:

https://boris.unibe.ch/id/eprint/76344

Actions (login required)

Edit item Edit item
Provide Feedback