Serricchio, Mauro; Schmid, Adrien W; Steinmann, Michael Eduard; Sigel, Erwin; Rauch, Monika; Julkowska, Daria; Bonnefoy, Serge; Fort, Cécile; Bastin, Philippe; Bütikofer, Peter (2015). Flagellar membranes are rich in raft-forming phospholipids. Biology open BiO, 4(9), pp. 1143-1153. Company of Biologists 10.1242/bio.011957
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The observation that the membranes of flagella are enriched in sterols and sphingolipids has led to the hypothesis that flagella might be enriched in raft-forming lipids. However, a detailed lipidomic analysis of flagellar membranes is not available. Novel protocols to detach and isolate intact flagella from Trypanosoma brucei procyclic forms in combination with reverse-phase liquid chromatography high-resolution tandem mass spectrometry allowed us to determine the phospholipid composition of flagellar membranes relative to whole cells. Our analyses revealed that phosphatidylethanolamine, phosphatidylserine, ceramide and the sphingolipids inositol phosphorylceramide and sphingomyelin are enriched in flagella relative to whole cells. In contrast, phosphatidylcholine and phosphatidylinositol are strongly depleted in flagella. Within individual glycerophospholipid classes, we observed a preference for ether-type over diacyl-type molecular species in membranes of flagella. Our study provides direct evidence for a preferential presence of raft-forming phospholipids in flagellar membranes of T. brucei.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine |
UniBE Contributor: |
Serricchio, Mauro, Steinmann, Michael Eduard, Sigel, Erwin, Rauch, Monika, Bütikofer, Peter |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
2046-6390 |
Publisher: |
Company of Biologists |
Language: |
English |
Submitter: |
Barbara Franziska Järmann-Bangerter |
Date Deposited: |
04 Mar 2016 08:10 |
Last Modified: |
05 Dec 2022 14:52 |
Publisher DOI: |
10.1242/bio.011957 |
PubMed ID: |
26276100 |
Uncontrolled Keywords: |
Flagella; Lipid rafts; Mass spectrometry; Membrane lipids; Sphingolipids; Trypanosoma brucei |
BORIS DOI: |
10.7892/boris.76344 |
URI: |
https://boris.unibe.ch/id/eprint/76344 |
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