T- and B-cell responses and previous exposure to hepatitis B virus in 'anti-HBc alone' patients.

Wang, Qixia; Sachse, Karl Philip; Semmo, M; Lokhande, Megha; Montani, Matteo; Dufour, Jean-François; Zoulim, F; Klenerman, Paul; Semmo, Nasser (2015). T- and B-cell responses and previous exposure to hepatitis B virus in 'anti-HBc alone' patients. Journal of viral hepatitis, 22(12), pp. 1068-1078. Blackwell Science 10.1111/jvh.12428

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A serologic response to hepatitis B virus (HBV) defined as 'anti-HBc alone' is commonly observed, but its significance remains unclear. This study aimed to define the relationship between 'anti-HBc alone' serostatus and HBV infection, including HBV-specific T- and B-cell memory responses. We enrolled 31 'anti-HBc alone' patients. Total HBV DNA and cccDNA were tested by nested polymerase chain reaction (PCR) analysis in liver samples from 22 'anti-HBc alone' patients vs controls (chronic or resolved HBV infection), followed by HBsAg/HBcAg immunohistochemical (IHC) staining. IFN-γ secretion by HBV-specific T cells was compared in individuals who were 'anti-HBc alone' (n = 27), resolved HBV (n = 21), chronic HBV (n = 24) and 12 healthy controls using enzyme-linked immunospot (ELISpot) assays. An HBsAg-IgG B-cell ELISpot assay was performed in 'anti-HBc alone' patients before and after one dose of recombinant HBsAg vaccine. The majority (23/31, 74.2%) of the 'anti-HBc alone' individuals were co-infected with HCV. Infrequent intrahepatic total HBV DNA (2/22, 9.1%) and cccDNA (1/22, 4.5%) were detected in biopsies; HBsAg and HBcAg IHC staining was negative. HBV-specific T-cell responses were similar between 'anti-HBc alone' individuals and HBV resolvers. Circulating HBV-memory B-cell responses were detected in all 'anti-HBc alone' individuals, consistent with an HBsAg-specific memory pool. After one HBV vaccine dose, increased anti-HBs antibody levels were observed, accompanied by an expansion of HBsAg-specific memory B cells (P = 0.0226). 'Anti-HBc alone' individuals showed HBV-specific T-cell and memory B-cell responses typical of previous viral exposure and protective memory, suggesting a resolved infection.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Hepatologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Hepatologie

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Hepatology
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Wang, Qixia; Sachse, Karl Philip; Lokhande, Megha; Montani, Matteo; Dufour, Jean-François; Klenerman, Paul and Semmo, Nasser

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

1352-0504

Publisher:

Blackwell Science

Language:

English

Submitter:

Lilian Karin Smith-Wirth

Date Deposited:

23 Mar 2016 08:19

Last Modified:

11 Sep 2017 15:56

Publisher DOI:

10.1111/jvh.12428

PubMed ID:

26075501

Uncontrolled Keywords:

T cells; anti-HBc alone; hepatitis B; memory B cells

BORIS DOI:

10.7892/boris.77702

URI:

https://boris.unibe.ch/id/eprint/77702

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