TAC102 Is a Novel Component of the Mitochondrial Genome Segregation Machinery in Trypanosomes

Trikin, Roman; Doiron, Nicholas; Hoffmann, Anneliese; Haenni, Beat; Jakob, Martin; Schnaufer, Achim; Schimanski, Bernd; Zuber, Benoît; Ochsenreiter, Torsten (2016). TAC102 Is a Novel Component of the Mitochondrial Genome Segregation Machinery in Trypanosomes. PLoS pathogens, 12(5), e1005586. Public Library of Science 10.1371/journal.ppat.1005586

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Trypanosomes show an intriguing organization of their mitochondrial DNA into a catenated network, the kinetoplast DNA (kDNA). While more than 30 proteins involved in kDNA replication have been described, only few components of kDNA segregation machinery are currently known. Electron microscopy studies identified a high-order structure, the tripartite attachment complex (TAC), linking the basal body of the flagellum via the mitochondrial membranes to the kDNA. Here we describe TAC102, a novel core component of the TAC, which is essential for proper kDNA segregation during cell division. Loss of TAC102 leads to mitochondrial genome missegregation but has no impact on proper organelle biogenesis and segregation. The protein is present throughout the cell cycle and is assembled into the newly developing TAC only after the pro-basal body has matured indicating a hierarchy in the assembly process. Furthermore, we provide evidence that the TAC is replicated de novo rather than using a semi-conservative mechanism. Lastly, we demonstrate that TAC102 lacks an N-terminal mitochondrial targeting sequence and requires sequences in the C-terminal part of the protein for its proper localization.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
08 Faculty of Science > Department of Biology > Institute of Cell Biology
09 Interdisciplinary Units > Microscopy Imaging Center MIC

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Trikin, Roman; Doiron, Nicholas; Hoffmann, Anneliese; Haenni, Beat; Jakob, Martin; Schimanski, Bernd; Zuber, Benoît and Ochsenreiter, Torsten

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1553-7366

Publisher:

Public Library of Science

Language:

English

Submitter:

Benoît Zuber

Date Deposited:

01 Jun 2016 14:54

Last Modified:

14 Feb 2019 18:05

Publisher DOI:

10.1371/journal.ppat.1005586

PubMed ID:

27168148

BORIS DOI:

10.7892/boris.82719

URI:

https://boris.unibe.ch/id/eprint/82719

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