Optimized methods for extracting circulating small RNAs from long-term stored equine samples.

Unger, Lucia; Fouché, Nathalie Elisa; Leeb, Tosso; Gerber, Vinzenz; Pacholewska, Alicja Elzbieta (2016). Optimized methods for extracting circulating small RNAs from long-term stored equine samples. Acta Veterinaria Scandinavica, 58(1), p. 44. BioMed Central Ltd. 10.1186/s13028-016-0224-5

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Circulating miRNAs in body fluids, particularly serum, are promising candidates for future routine biomarker profiling in various pathologic conditions in human and veterinary medicine. However, reliable standardized methods for miRNA extraction from equine serum and fresh or archived whole blood are sorely lacking. We systematically compared various miRNA extraction methods from serum and whole blood after short and long-term storage without addition of RNA stabilizing additives prior to freezing. Time of storage at room temperature prior to freezing did not affect miRNA quality in serum. Furthermore, we showed that miRNA of NGS-sufficient quality can be recovered from blood samples after >10 years of storage at -80 °C. This allows retrospective analyses of miRNAs from archived samples.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Unger, Lucia; Fouché, Nathalie Elisa; Leeb, Tosso; Gerber, Vinzenz and Pacholewska, Alicja Elzbieta

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

1751-0147

Publisher:

BioMed Central Ltd.

Language:

English

Submitter:

Tosso Leeb

Date Deposited:

12 Jul 2016 15:53

Last Modified:

06 Jan 2017 17:21

Publisher DOI:

10.1186/s13028-016-0224-5

PubMed ID:

27356979

Uncontrolled Keywords:

EDTA; Horse; RNA extraction; Serum; Small RNA; miRNA; microRNA

BORIS DOI:

10.7892/boris.84124

URI:

https://boris.unibe.ch/id/eprint/84124

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