Rau, Mark; Nicolussi, Simon; Chicca, Andrea; Gertsch, Jürg (2016). Assay of Endocannabinoid Uptake. In: Maccarrone, Mauro (ed.) Endocannabinoid Signaling. Methods and Protocols. Methods in Molecular Biology: Vol. 1412 (pp. 191-203). New York: Springer 10.1007/978-1-4939-3539-0_20
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Endocannabinoids at physiological concentrations are crossing cellular membranes by facilitated diffusion, a process that can be studied by measuring transport kinetics. Here, we describe a radiosubstrate-based assay using arachidonoyl[1-(3)H]ethanolamine or arachidonoyl[1,2,3-(3)H]glycerol to measure the cellular endocannabinoid uptake in a three-phase assay with human U937 cells.
Item Type: |
Book Section (Book Chapter) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Faculty Institutions > NCCR TransCure 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine |
UniBE Contributor: |
Rau, Mark, Nicolussi, Simon, Chicca, Andrea, Gertsch, Jürg |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
1064-3745 |
ISBN: |
978-1-4939-3539-0 |
Series: |
Methods in Molecular Biology |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Valentina Rossetti |
Date Deposited: |
15 Sep 2016 11:09 |
Last Modified: |
05 Dec 2022 14:58 |
Publisher DOI: |
10.1007/978-1-4939-3539-0_20 |
PubMed ID: |
27245905 |
Uncontrolled Keywords: |
2-Arachidonoylglycerol; Anandamide; Endocannabinoid uptake |
BORIS DOI: |
10.7892/boris.87536 |
URI: |
https://boris.unibe.ch/id/eprint/87536 |
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