Assay of Endocannabinoid Uptake

Rau, Mark; Nicolussi, Simon; Chicca, Andrea; Gertsch, Jürg (2016). Assay of Endocannabinoid Uptake. In: Maccarrone, Mauro (ed.) Endocannabinoid Signaling. Methods and Protocols. Methods in Molecular Biology: Vol. 1412 (pp. 191-203). New York: Springer 10.1007/978-1-4939-3539-0_20

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Endocannabinoids at physiological concentrations are crossing cellular membranes by facilitated diffusion, a process that can be studied by measuring transport kinetics. Here, we describe a radiosubstrate-based assay using arachidonoyl[1-(3)H]ethanolamine or arachidonoyl[1,2,3-(3)H]glycerol to measure the cellular endocannabinoid uptake in a three-phase assay with human U937 cells.

Item Type:

Book Section (Book Chapter)

Division/Institute:

04 Faculty of Medicine > Other Institutions > NCCR TransCure
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Rau, Mark; Nicolussi, Simon; Chicca, Andrea and Gertsch, Jürg

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1064-3745

ISBN:

978-1-4939-3539-0

Series:

Methods in Molecular Biology

Publisher:

Springer

Language:

English

Submitter:

Valentina Rossetti

Date Deposited:

15 Sep 2016 11:09

Last Modified:

01 May 2017 15:27

Publisher DOI:

10.1007/978-1-4939-3539-0_20

PubMed ID:

27245905

Uncontrolled Keywords:

2-Arachidonoylglycerol; Anandamide; Endocannabinoid uptake

BORIS DOI:

10.7892/boris.87536

URI:

https://boris.unibe.ch/id/eprint/87536

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