Pathway Diversity in the Self-Assembly of DNA-Derived Bioconjugates

Vyborna, Yuliia; Vybornyi, Mykhailo; Häner, Robert (2016). Pathway Diversity in the Self-Assembly of DNA-Derived Bioconjugates. Bioconjugate chemistry, 27(11), pp. 2755-2761. American Chemical Society 10.1021/acs.bioconjchem.6b00517

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The pathway diversity of the self-assembly of amphiphilic DNA-pyrene conjugates is described. The hydrophobic pyrene units drive the self-assembly of the anionic oligomers in an aqueous environment into ribbon-shaped, DNA-grafted supramolecular polymers. Isothermal mixing of two types of sorted ribbons, each of which contains only one kind of two complementary oligonucleotides, results in the formation of tight networks. Thermal disassembly of these kinetically trapped networks and subsequent re-assembly of the liberated components leads to mixed supramolecular polymers, which now contain both types of oligonucleotides. The scrambling of the oligonucleotides prevents the interaction between ribbons and, thus, network formation. The results show that a high local density of DNA strands in linear arrays favors hybridization among sorted polymers, whereas hybridization among mixed arrays is prevented. The lack of DNA hybridization among mixed ribbons is ascribed to the electrostatic repulsion between identical, hence non-complementary, oligonucleotides. The findings highlight the importance of kinetically trapped states on the structural and functional properties of supramolecular polymers containing orthogonal self-assembly motifs.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Vyborna, Yuliia; Vybornyi, Mykhailo and Häner, Robert

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

1043-1802

Publisher:

American Chemical Society

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Robert Häner

Date Deposited:

04 Jan 2017 15:32

Last Modified:

15 Jul 2019 15:19

Publisher DOI:

10.1021/acs.bioconjchem.6b00517

PubMed ID:

27728972

BORIS DOI:

10.7892/boris.90082

URI:

https://boris.unibe.ch/id/eprint/90082

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