Lesche, Dorothea; Geyer, Roland; Lienhard, Daniel; Nakas, Christos T.; Diserens, Gaëlle; Vermathen, Peter; Leichtle, Alexander Benedikt (2016). Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome. Metabolomics, 12(10), p. 159. Springer 10.1007/s11306-016-1109-3
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BACKGROUND
Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs.
AIM
Determine whether routinely used plasma centrifugation protocols (1500×g 10 min; 3000×g 5 min) influence non-targeted metabolomic analyses.
METHODS
Nuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements.
RESULTS
Besides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols.
CONCLUSION
Already minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies.
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