Comprehensive assessment of tumour budding on cytokeratin stains in colorectal cancer.

Rieger, Gregor; Kölzer, Viktor; Dawson, Heather; Berger, Martin Dave; Hädrich, Marion; Inderbitzin, Daniel; Lugli, Alessandro; Zlobec, Inti (2017). Comprehensive assessment of tumour budding on cytokeratin stains in colorectal cancer. Histopathology, 70(7), pp. 1044-1051. Blackwell Scientific Publications 10.1111/his.13164

[img]
Preview
Text
Comprehensive assessment of tumour budding on cytokeratin stains in colorectal cancer..pdf - Accepted Version
Available under License Publisher holds Copyright.

Download (623kB) | Preview

BACKGROUND Tumour budding in colorectal cancer (CRC) is a recognized prognostic parameter. Aim of this study is to address the use of cytokeratin immunostaining for visualization and scoring of tumour buds. METHODS Ten hotspots (0.238 mm(2) ) of peritumoural (PTB) and intratumoural (ITB) budding were evaluated in surgical resections from 215 patients. The budding counts in the 10 densest regions anywhere in the tumour were combined into an overall tumour budding (OTB) score. The PTB, ITB and OTB hotspot with the maximum budding count was then evaluated. Finally, continuous and cut-off values of 10 buds/HPF (PTB10HPF ), 5 buds/HPF (ITB10HPF ) and 8 buds/HPF (OTB10HPF ) were used to categorize budding counts into low/high-grade scores. RESULTS All budding scores were highly correlated. PTB and ITB counts were associated with many clinicopathological features including tumour stage, lymph node and distant metastasis, venous and lymphovascular invasion and disease-free survival (DFS) (all p<0.05). Analyses of OTB counts recapitulated these associations, including a lower DFS with a greater number of tumour buds (p=0.0309; HR (95%CI): 1.032 (1.003-1.062)). One OTB hotspot performed similarly as ten OTB hotspots in terms of relationship with outcome. These statistical significances were largely lost when cut-offs were applied to PTB, ITB or OTB counts. CONCLUSIONS OTB count in a single hotspot on cytokeratin-stained CRC tissue sections is a fast and reliable prognostic scoring system for the assessment of tumour budding. This approach should be considered in future studies. This article is protected by copyright. All rights reserved.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology > Autopsy
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie

04 Faculty of Medicine > Service Sector > Institute of Pathology > Clinical Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Medical Oncology

UniBE Contributor:

Rieger, Gregor; Kölzer, Viktor; Dawson, Heather; Berger, Martin Dave; Hädrich, Marion; Inderbitzin, Daniel; Lugli, Alessandro and Zlobec, Inti

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0309-0167

Publisher:

Blackwell Scientific Publications

Language:

English

Submitter:

Lilian Karin Smith-Wirth

Date Deposited:

01 Feb 2017 14:19

Last Modified:

07 Jan 2018 02:30

Publisher DOI:

10.1111/his.13164

PubMed ID:

28061021

Uncontrolled Keywords:

colorectal cancer; pathology; precision medicine; prognosis

BORIS DOI:

10.7892/boris.94498

URI:

https://boris.unibe.ch/id/eprint/94498

Actions (login required)

Edit item Edit item
Provide Feedback