Heterogeneous antigenic properties of the porcine reproductive and respiratory syndrome virus nucleocapsid.

Rappe, Julie Christiane Françoise; Garcia Nicolas, Obdulio; Flückiger, Franziska; Thür, Barbara; Hofmann, Martin A; Summerfield, Artur; Ruggli, Nicolas (2016). Heterogeneous antigenic properties of the porcine reproductive and respiratory syndrome virus nucleocapsid. Veterinary research, 47(1), p. 117. Editions scientifiques Elsevier 10.1186/s13567-016-0399-9

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Porcine reproductive and respiratory syndrome virus (PRRSV) is an arterivirus responsible for a widespread contagious disease of domestic pigs with high economic impact. Switzerland is one of the rare PRRSV-free countries in Europe, although sporadic outbreaks have occurred in the past. The PRRSV isolate IVI-1173 from the short outbreak in Switzerland in 2012 was entirely sequenced, and a functional full-length cDNA clone was constructed. Genetic and antigenic characterization of IVI-1173 revealed the importance of amino acid 90 of the nucleocapsid protein N as part of a conformational epitope. IVI-1173 was not detected by SDOW17, a monoclonal antibody against N widely used to detect PRRSV-infected cells. Substitution of alanine at position 90 of N [N(A90)] with a threonine [N(T90)] restored reactivity of vIVI1173-N(T90) to SDOW17 completely. The relevance of this amino acid for the conformational SDOW17 epitope of PRRSV N was further confirmed by the opposite substitution in a functional cDNA clone of the genotype 2 isolate RVB-581. Finally, N proteins from ten genotype 1 strains differing from threonine at position 90 were analysed for reactivity with SDOW17. N(A90) totally disrupted or severely affected the epitope in 7 out of 8 strains tested. Based on these findings, 225 genotype 1 strains were screened for the prevalence of N(A90). N(A90) is rare in classical subtype 1 and in subtype 3 strains, but is frequent in Russian subtype 1 (70%) and in subtype 2 (45%) isolates. In conclusion, this study highlights the variable antigenic properties of N among genotype 1 PRRSV strains.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Rappe, Julie Christiane Françoise; Garcia Nicolas, Obdulio; Thür, Barbara; Summerfield, Artur and Ruggli, Nicolas

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0928-4249

Publisher:

Editions scientifiques Elsevier

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

01 Jun 2017 12:43

Last Modified:

04 Jun 2017 02:15

Publisher DOI:

10.1186/s13567-016-0399-9

PubMed ID:

27871316

BORIS DOI:

10.7892/boris.96161

URI:

https://boris.unibe.ch/id/eprint/96161

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