Characterization of the tobacco eIF-4A gene family

Owttrim, George W; Mandel, Therese; Trachsel, Hans; Thomas, Adri AM; Kuhlemeier, Cris (1994). Characterization of the tobacco eIF-4A gene family. Plant molecular biology, 26(6), pp. 1747-1757. Springer 10.1007/BF00019489

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Characterization of cDNAs encoding eukaryotic translation initiation factor 4A (eIF-4A) indicates the expression of a minimum of ten related genes in tobacco leaf cells. The ten groups fall into two gene families, NeIF-4A2 and NeIF-4A3. The majority of the cDNAs exhibit significant sequence similarity to the NeIF-4A2 family at both the DNA and deduced amino acid levels. Northern analysis using specific probes indicates variable expression of four family members in various tobacco organs. Western analysis, using an anti-tobacco eIF-4A polyclonal antibody, reveals a complex pattern of immunologically related polypeptides of approximately 46 kDa. Subcellular fractionation suggests that at least one eIF-4A-related polypeptide is located in the chloroplast where it is ribosome-associated.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Mandel, Therese; Trachsel, Hans and Kuhlemeier, Cris

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
500 Science > 580 Plants (Botany)

ISSN:

0167-4412

Publisher:

Springer

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

05 Jul 2017 09:52

Last Modified:

05 Jul 2017 09:52

Publisher DOI:

10.1007/BF00019489

Uncontrolled Keywords:

cDNA gene family, NeIF-4A, RNA helicase, translation initiation factor, Nicotiana

BORIS DOI:

10.7892/boris.97867

URI:

https://boris.unibe.ch/id/eprint/97867

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