Owttrim, George W; Mandel, Therese; Trachsel, Hans; Thomas, Adri AM; Kuhlemeier, Cris (1994). Characterization of the tobacco eIF-4A gene family. Plant molecular biology, 26(6), pp. 1747-1757. Springer 10.1007/BF00019489
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Characterization of cDNAs encoding eukaryotic translation initiation factor 4A (eIF-4A) indicates the expression of a minimum of ten related genes in tobacco leaf cells. The ten groups fall into two gene families, NeIF-4A2 and NeIF-4A3. The majority of the cDNAs exhibit significant sequence similarity to the NeIF-4A2 family at both the DNA and deduced amino acid levels. Northern analysis using specific probes indicates variable expression of four family members in various tobacco organs. Western analysis, using an anti-tobacco eIF-4A polyclonal antibody, reveals a complex pattern of immunologically related polypeptides of approximately 46 kDa. Subcellular fractionation suggests that at least one eIF-4A-related polypeptide is located in the chloroplast where it is ribosome-associated.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine 08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development 08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) |
UniBE Contributor: |
Mandel, Therese, Trachsel, Hans, Kuhlemeier, Cris |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health 500 Science > 580 Plants (Botany) |
ISSN: |
0167-4412 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Peter Alfred von Ballmoos-Haas |
Date Deposited: |
05 Jul 2017 09:52 |
Last Modified: |
05 Dec 2022 15:04 |
Publisher DOI: |
10.1007/BF00019489 |
Uncontrolled Keywords: |
cDNA gene family, NeIF-4A, RNA helicase, translation initiation factor, Nicotiana |
BORIS DOI: |
10.7892/boris.97867 |
URI: |
https://boris.unibe.ch/id/eprint/97867 |
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