Cupiennin 1a exhibits a remarkably broad, non-stereospecific cytolytic activity on bacteria, protozoan parasites, insects, and human cancer cells

Kuhn-Nentwig, Lucia; Willems, Jean; Seebeck, Thomas; Shalaby, Tarek; Kaiser, Marcel; Nentwig, Wolfgang (2011). Cupiennin 1a exhibits a remarkably broad, non-stereospecific cytolytic activity on bacteria, protozoan parasites, insects, and human cancer cells. Amino acids, 40(1), pp. 69-76. Wien: Springer Vienna 10.1007/s00726-009-0471-0

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Cupiennin 1a, a cytolytic peptide isolated from the venom of the spider Cupiennius salei, exhibits broad membranolytic activity towards bacteria, trypanosomes, and plasmodia, as well as human blood and cancer cells. In analysing the cytolytic activity of synthesised all-d- and all-l-cupiennin 1a towards pro- and eukaryotic cells, a stereospecific mode of membrane destruction could be excluded. The importance of negatively charged sialic acids on the outer leaflet of erythrocytes for the binding and haemolytic activity of l-cupiennin 1a was demonstrated. Reducing the overall negative charges of erythrocytes by partially removing their sialic acids or by protecting them with tri- or pentalysine results in reduced haemolytic activity of the peptide.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Community Ecology
08 Faculty of Science > Department of Biology > Institute of Cell Biology
08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Kuhn-Nentwig, Lucia; Willems, Johannes; Seebeck, Thomas and Nentwig, Wolfgang

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)
500 Science > 580 Plants (Botany)
500 Science > 540 Chemistry

ISSN:

0939-4451

Publisher:

Springer Vienna

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:27

Last Modified:

23 Oct 2019 17:06

Publisher DOI:

10.1007/s00726-009-0471-0

PubMed ID:

20140690

BORIS DOI:

10.7892/boris.9819

URI:

https://boris.unibe.ch/id/eprint/9819 (FactScience: 215613)

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