The role of eukaryotic rRNA expansion segments in protein synthesis

Ganapathy Shankar, Vaishnavi (25 September 2017). The role of eukaryotic rRNA expansion segments in protein synthesis (Unpublished). In: Riboclub Annual Meeting. Hôtel Chéribourg, Magog, Canada. 25.-28.09.2017.

The ribosome translates the information encoded by mRNA into sequence of amino acids, thereby synthesizing all proteins required by the cell. The main functional core of the ribosome comprising the subunit interface sides, the peptidyl transferase centre (PTC) and the decoding site, are conserved across all three domains of life. However, compared to the prokaryotic ribosome (70S), the eukaryotic one (80S) is about 40% larger and contains additional ribosomal proteins as well as additional ribosomal RNA (rRNA) elements, the expansion segments (ES) (1,2). Eukaryotic ES are mainly located at the periphery of the ribosome though the precise functions remain uncertain. However there is accumulating evidence that ES play a role in translation possibly by recruiting regulatory factors to the ribosome (3). To unravel the function of ES in translation specifically, ES7S and ES27L, these two ES were deleted from the ribosomes in the model organism S. cerevisiae, and were studied for growth phenotypes and functionality in translation and subsets thereof. Preliminary results show a serious growth phenotype upon ES7S deletion, lethality for ES27L deletion and no growth phenotype for strains carrying ribosomes with a section of ES27L deleted (ES27Lb deletion). Although the translation rate is greatly slowed down in ES7S mutants, there appears to be no biogenesis defects.

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