Seiler, Theo G.; Engler, Marleen; Beck, Eric; Birngruber, Reginald; Kochevar, Irene E (2017). Interface Bonding With Corneal Crosslinking (CXL) After LASIK Ex Vivo. Investigative ophthalmology & visual science, 58(14), pp. 6292-6298. Association for Research in Vision and Ophthalmology 10.1167/iovs.17-22426
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Purpose
Interface bonding with corneal crosslinking (CXL) after LASIK using two different photosensitizers was studied ex vivo.
Methods
A LASIK flap was created in enucleated rabbit eyes using a femtosecond laser. After the dissection, CXL was performed to seal the interface. In one group interface CXL was performed using rose bengal and green light, whereas in a second group riboflavin and UV-A light was used. In both groups irradiance, radiant exposure, dye concentration, and imbibition time was varied. In a control group, LASIK only was performed. After the procedures, the maximal shear-force required to separate the flap from the stroma was measured. Additionally, corneal transmission spectra were recorded.
Results
Optimized parameters for rose bengal/green-light bonding lead to a 2.1-fold increase in shear-force compared with untreated control eyes (P < 0.01). The optimal parameter combination was: irradiance of 180 mW/cm2 for 14 minutes (total radiant exposure 150 J/cm2), rose bengal concentration 0.1%, and an imbibition time of 2 minutes. Optimized riboflavin/UV-A light parameters were 0.5% for 2 minutes with a radiant exposure of 8.1 J/cm2 obtained by an irradiance of 30 mW/cm2 for 4.5 minutes. These optimized parameters lead to a 2-fold increase compared with untreated control eyes (P < 0.01). Optical transmission experiments suggest safety for more posterior structures.
Conclusions
Based on ex-vivo results, interface bonding after LASIK using crosslinking with either rose bengal or riboflavin increases the adhesion between flap and stromal bed. In vivo trials are needed to evaluate the temporal evolution of the effect.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology |
UniBE Contributor: |
Seiler, Günter Theodor Michael |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0146-0404 |
Publisher: |
Association for Research in Vision and Ophthalmology |
Language: |
English |
Submitter: |
Günter Theodor Michael Seiler |
Date Deposited: |
26 Feb 2018 15:16 |
Last Modified: |
05 Dec 2022 15:09 |
Publisher DOI: |
10.1167/iovs.17-22426 |
PubMed ID: |
29242903 |
BORIS DOI: |
10.7892/boris.108523 |
URI: |
https://boris.unibe.ch/id/eprint/108523 |
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