Pizzagalli, Diego Ulisse; Farsakoglu, Yagmur; Palomino-Segura, Miguel; Palladino, Elisa; Sintes, Jordi; Marangoni, Francesco; Mempel, Thorsten R; Koh, Wan Hon; Murooka, Thomas T; Thelen, Flavian; Stein, Jens V; Pozzi, Giuseppe; Thelen, Marcus; Krause, Rolf; Gonzalez, Santiago Fernandez (2018). Leukocyte Tracking Database, a collection of immune cell tracks from intravital 2-photon microscopy videos. Scientific data, 5(180129), p. 180129. Nature Publishing Group 10.1038/sdata.2018.129
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Recent advances in intravital video microscopy have allowed the visualization of leukocyte behavior in vivo, revealing unprecedented spatiotemporal dynamics of immune cell interaction. However, state-of-the-art software and methods for automatically measuring cell migration exhibit limitations in tracking the position of leukocytes over time. Challenges arise both from the complex migration patterns of these cells and from the experimental artifacts introduced during image acquisition. Additionally, the development of novel tracking tools is hampered by the lack of a sound ground truth for algorithm validation and benchmarking. Therefore, the objective of this work was to create a database, namely LTDB, with a significant number of manually tracked leukocytes. Broad experimental conditions, sites of imaging, types of immune cells and challenging case studies were included to foster the development of robust computer vision techniques for imaging-based immunological research. Lastly, LTDB represents a step towards the unravelling of biological mechanisms by video data mining in systems biology.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute 09 Interdisciplinary Units > Microscopy Imaging Center (MIC) |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Thelen, Flavian |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
2052-4463 |
Publisher: |
Nature Publishing Group |
Language: |
English |
Submitter: |
Ursula Zingg-Zünd |
Date Deposited: |
27 Feb 2019 12:13 |
Last Modified: |
05 Dec 2022 15:24 |
Publisher DOI: |
10.1038/sdata.2018.129 |
PubMed ID: |
30015806 |
BORIS DOI: |
10.7892/boris.124293 |
URI: |
https://boris.unibe.ch/id/eprint/124293 |
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