Bachmann, Fabio; Blaser, Lea; Haschke, Manuel Martin; Krähenbühl, Stephan; Duthaler, Urs (2020). Development and validation of an LC-MS/MS method for the bioanalysis of the major metamizole metabolites in human plasma. Bioanalysis, 12(3), pp. 175-189. Future Science 10.4155/bio-2019-0251
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Aim: Metamizole is a frequently used antipyretic and analgesic prodrug, yet its pharmacokinetics has not been thoroughly studied in infants and with coadministered medications. Thus, an LC-MS/MS method was developed to quantify the four major metamizole metabolites in human plasma. Methodology: Pre- and postcolumn infusion was installed to enable robust analyte retention and electrospray ionization following deproteinization of plasma samples. Results: The method was linear (R > 0.996), accurate (93.1-106.0%) and precise (≤12.7%). Mean recovery was more than 91.8% and ion suppression less than 13.1% for all analytes. Pharmacokinetic profiles were reproducible after 4 years at -80°C except for the formylated metabolite (-22.2%). Conclusion: The method fulfilled pertinent criteria of validation guidelines and required only little sample volume. The method therefore qualifies for metamizole analyses in children.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of General Internal Medicine (DAIM) > Clinic of General Internal Medicine |
UniBE Contributor: |
Haschke, Manuel Martin |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1757-6180 |
Publisher: |
Future Science |
Language: |
English |
Submitter: |
Tobias Tritschler |
Date Deposited: |
10 Mar 2020 14:33 |
Last Modified: |
05 Dec 2022 15:37 |
Publisher DOI: |
10.4155/bio-2019-0251 |
PubMed ID: |
32052638 |
Uncontrolled Keywords: |
LC–MS/MS bioanalysis dipyrone method validation pediatrics pharmacokinetics |
BORIS DOI: |
10.7892/boris.140950 |
URI: |
https://boris.unibe.ch/id/eprint/140950 |