A de novo chromosome‐level genome assembly of Coregonus sp. “Balchen” : one representative of the Swiss Alpine whitefish radiation

De-Kayne, Rishi; Zoller, Stefan; Feulner, Philine G. D. (2020). A de novo chromosome‐level genome assembly of Coregonus sp. “Balchen” : one representative of the Swiss Alpine whitefish radiation. Molecular ecology resources, 20(4), pp. 1093-1109. Wiley 10.1111/1755-0998.13187

Preview	Text De-Kayne et al. 2020_MolEcolRes_A de novo chromosome-level genome assembly of Coregonus sp Balchen.pdf - Accepted Version Available under License Publisher holds Copyright. Download (20MB) | Preview
Preview	Text De-Kayne et al. 2020_MolEcolRes_A de novo chromosome-level genome assembly of Coregonus sp Balchen.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (2MB) | Preview

Salmonids are of particular interest to evolutionary biologists due to their incredible diversity of life-history strategies and the speed at which many salmonid species have diversified. In Switzerland alone, over 30 species of Alpine whitefish from the subfamily Coregoninae have evolved since the last glacial maximum, with species exhibiting a diverse range of morphological and behavioural phenotypes. This, combined with the whole genome duplication which occurred in the ancestor of all salmonids, makes the Alpine whitefish radiation a particularly interesting system in which to study the genetic basis of adaptation and speciation and the impacts of ploidy changes and subsequent rediploidization on genome evolution. Although well curated genome assemblies exist for many species within Salmonidae, genomic resources for the subfamily Coregoninae are lacking. To assemble a whitefish reference genome, we carried out PacBio sequencing from one wild-caught Coregonus sp. “Balchen” from Lake Thun to ~90x coverage. PacBio reads were assembled independently using three different assemblers, Falcon, Canu and wtdbg2 and subsequently scaffolded with additional Hi-C data. All three assemblies were highly contiguous, had strong synteny to a previously published Coregonus linkage map, and when mapping additional short-read data to each of the assemblies, coverage was fairly even across most chromosome-scale scaffolds. Here, we present the first de novo genome assembly for the Salmonid subfamily Coregoninae. The final 2.2 Gb wtdbg2 assembly included 40 scaffolds, an N50 of 51.9 Mb, and was 93.3% complete for BUSCOs. The assembly consisted of ~52% TEs and contained 44,525 genes.

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