Binder, Florian; Ryll, René; Drewes, Stephan; Jagdmann, Sandra; Reil, Daniela; Hiltbrunner, Melanie; Rosenfeld, Ulrike M.; Imholt, Christian; Jacob, Jens; Heckel, Gerald; Ulrich, Rainer G. (2020). Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment. Pathogens, 9(7) MDPI AG 10.3390/pathogens9070548
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The S segment of bank vole (Clethrionomys glareolus)-associated Puumala orthohantavirus (PUUV) contains two overlapping open reading frames coding for the nucleocapsid (N) and a non-structural (NSs) protein. To identify the influence of bank vole population dynamics on PUUV S segment sequence evolution and test for spillover infections in sympatric rodent species, during 2010–2014, 883 bank voles, 357 yellow-necked mice (Apodemus flavicollis), 62 wood mice
(A. sylvaticus), 149 common voles (Microtus arvalis) and 8 field voles (M. agrestis) were collected in Baden-Wuerttemberg and North Rhine-Westphalia, Germany. In total, 27.9% and 22.3% of bank voles were positive for PUUV-reactive antibodies and PUUV-specific RNA, respectively. One of eight field voles was PUUV RNA-positive, indicating a spillover infection, but none of the other species showed evidence of PUUV infection. Phylogenetic and isolation-by-distance analyses demonstrated a spatial clustering of PUUV S segment sequences. In the hantavirus outbreak years 2010 and 2012, PUUV RNA prevalence was higher in our study regions compared to non-outbreak years 2011, 2013 and 2014. NSs amino acid and nucleotide sequence types showed temporal and/or local variation, whereas the N protein was highly conserved in the NSs overlapping region and, to a lower rate, in the N alone coding part.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) 08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Population Genetics |
UniBE Contributor: |
Hiltbrunner, Melanie, Heckel, Gerald |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
2076-0817 |
Publisher: |
MDPI AG |
Language: |
English |
Submitter: |
Susanne Holenstein |
Date Deposited: |
01 Sep 2020 16:30 |
Last Modified: |
23 Dec 2022 09:42 |
Publisher DOI: |
10.3390/pathogens9070548 |
PubMed ID: |
32650456 |
BORIS DOI: |
10.7892/boris.146071 |
URI: |
https://boris.unibe.ch/id/eprint/146071 |
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