McCloskey, Molly C; Kasap, Pelin; Ahmad, S Danial; Su, Shiuan-Haur; Chen, Kaihua; Mansouri, Mehran; Ramesh, Natalie; Nishihara, Hideaki; Belyaev, Yury; Abhyankar, Vinay V; Begolo, Stefano; Singer, Benjamin H; Webb, Kevin F; Kurabayashi, Katsuo; Flax, Jonathan; Waugh, Richard E; Engelhardt, Britta; McGrath, James L (2022). The Modular μSiM: a Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro. Advanced healthcare materials, 11(18), e2200804. Wiley 10.1002/adhm.202200804
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Adv_Healthcare_Materials_-_2022_-_McCloskey_-_The_Modular_SiM_a_Mass_Produced_Rapidly_Assembled_and_Reconfigurable.pdf - Accepted Version Available under License Publisher holds Copyright. Download (4MB) | Preview |
Advanced in vitro tissue chip models can reduce and replace animal experimentation and may eventually support "on-chip" clinical trials. To realize this potential, however, tissue chip platforms must be both mass-produced and reconfigurable to allow for customized design. To address these unmet needs, we introduce an extension of our μSiM (microdevice featuring a silicon-nitride membrane) platform. The modular μSiM (m-μSiM) uses mass-produced components to enable rapid assembly and reconfiguration by laboratories without knowledge of microfabrication. We demonstrate the utility of the m-μSiM by establishing an hiPSC-derived blood-brain barrier (BBB) in bioengineering and non-engineering, brain barriers focused laboratories. We develop and validate in situ and sampling-based assays of small molecule diffusion as a measure of barrier function. BBB properties show excellent interlaboratory agreement and match expectations from literature, validating the m-μSiM as a platform for barrier models and demonstrating successful dissemination of components and protocols. We then demonstrate the ability to quickly reconfigure the m-μSiM for co-culture and immune cell transmigration studies through addition of accessories and/or quick exchange of components. Because the development of modified components and accessories is easily achieved, custom designs of the m-μSiM should be accessible to any laboratory desiring a barrier-style tissue chip platform. This article is protected by copyright. All rights reserved.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute 09 Interdisciplinary Units > Microscopy Imaging Center (MIC) |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Kasap, Pelin, Nishihara, Hideaki, Belyaev, Yury, Engelhardt, Britta |
Subjects: |
600 Technology > 610 Medicine & health 500 Science |
ISSN: |
2192-2659 |
Publisher: |
Wiley |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
29 Jul 2022 12:32 |
Last Modified: |
30 Jul 2023 00:25 |
Publisher DOI: |
10.1002/adhm.202200804 |
PubMed ID: |
35899801 |
Uncontrolled Keywords: |
blood-brain barrier membranes modular tissue chips vascular barriers |
BORIS DOI: |
10.48350/171632 |
URI: |
https://boris.unibe.ch/id/eprint/171632 |
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