Analysis of Biomechanical Response After Corneal Crosslinking with Different Fluence Levels in Porcine Corneas.

Fischinger, Isaak; Reifeltshammer, Sophia A; Seiler, Theo G; Nambiar, Malavika H; Komninou, Maria A; Büchler, Philippe; Wendelstein, Jascha; Langenbucher, Achim; Bolz, Matthias (2023). Analysis of Biomechanical Response After Corneal Crosslinking with Different Fluence Levels in Porcine Corneas. Current eye research, 48(8), pp. 719-723. Taylor & Francis 10.1080/02713683.2023.2205612

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PURPOSE

To evaluate corneal stiffening of porcine corneas induced by corneal crosslinking (CXL) with constant irradiance as a function of total fluence.

METHODS

Ninety corneas from freshly enucleated porcine eyes were divided into five groups of 18 eyes. Groups 1-4 underwent epi-off CXL using a dextran-based riboflavin solution and an irradiance of 18 mW/cm2, group 5 served as the control group. Groups 1 to 4 were treated with a total fluence of 20, 15, 10.8, and 5.4 J/cm2, respectively. Thereafter, biomechanical measurements were performed on 5 mm wide and 6 mm long strips using an uniaxial material tester. Pachymetry measurements were performed on each cornea.

RESULTS

At 10% strain, the stress was 76, 56, 52, and 31% higher in groups 1-4, respectively compared to the control group. The Young's modulus was 2.85 MPa for group 1, 2.53 MPa for group 2, 2.46 MPa for group 3, 2.12 MPa for group 4, and 1.62 MPa for the control group. The difference between groups 1 to 4 and the control group 5 were statistically significant (p = <0.001; p = <0.001; p = <0.001; p = 0.021). In addition, group 1 showed significantly more stiffening than group 4 (p = <0.001), no other significant differences were found. Pachymetry measurements revealed no statistically significant differences among the five groups.

CONCLUSION

Additional mechanical stiffening can be achieved by increasing the fluence of the CXL. There was no threshold detected up to 20 J/cm2. A higher fluence could compensate the weaker effect of accelerated or epi-on CXL procedures.

Item Type:

Journal Article (Original Article)

Division/Institute:

10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Computational Bioengineering
10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Musculoskeletal Biomechanics
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology
10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Seiler, Günter Theodor Michael, Nambiar, Malavika Harikrishnan, Büchler, Philippe

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1460-2202

Publisher:

Taylor & Francis

Language:

English

Submitter:

Pubmed Import

Date Deposited:

08 May 2023 12:51

Last Modified:

24 Jun 2024 11:42

Publisher DOI:

10.1080/02713683.2023.2205612

PubMed ID:

37144469

Uncontrolled Keywords:

Corneal crosslinking accelerated corneal biomechanics fluence keratoconus

BORIS DOI:

10.48350/182355

URI:

https://boris.unibe.ch/id/eprint/182355

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