Molecular profiling of osteoprogenitor cells reveals FOS as a master regulator of bone non-union.

La Manna, Federico; Hanhart, Daniel; Kloen, Peter; van Wijnen, Andre J; Thalmann, George; Kruithof-de Julio, Marianna; Chouvardas, Panagiotis (2023). Molecular profiling of osteoprogenitor cells reveals FOS as a master regulator of bone non-union. Gene, 874(147481), p. 147481. Elsevier 10.1016/j.gene.2023.147481

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Despite the advances in bone fracture treatment, a significant fraction of fracture patients will develop non-union. Most non-unions are treated with surgery since identifying the molecular causes of these defects is exceptionally challenging. In this study, compared with marrow bone, we generated a transcriptional atlas of human osteoprogenitor cells derived from healing callus and non-union fractures. Detailed comparison among the three conditions revealed a substantial similarity of callus and nonunion at the gene expression level. Nevertheless, when assayed functionally, they showed different osteogenic potential. Utilizing longitudinal transcriptional profiling of the osteoprogenitor cells, we identified FOS as a putative master regulator of non-union fractures. We validated FOS activity by profiling a validation cohort of 31 tissue samples. Our work identified new molecular targets for non-union classification and treatment while providing a valuable resource to better understand human bone healing biology.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Urologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Urologie

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Urology

UniBE Contributor:

La Manna, Federico, Hanhart, Daniel Walter, Thalmann, George, Kruithof-de Julio, Marianna, Chouvardas, Panagiotis

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0378-1119

Publisher:

Elsevier

Language:

English

Submitter:

Pubmed Import

Date Deposited:

15 May 2023 10:12

Last Modified:

13 May 2024 00:25

Publisher DOI:

10.1016/j.gene.2023.147481

PubMed ID:

37182560

Uncontrolled Keywords:

MSC RNA-sequencing master regulators non-union pathway analysis

BORIS DOI:

10.48350/182555

URI:

https://boris.unibe.ch/id/eprint/182555

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