Jayakumar, Remya; Dash, Manoj Kumar; Kumar, Pankaj; Sharma, Shiwakshi; Gulati, Saumya; Pandey, Akanksha; Cholke, Kaushavi; Fatima, Zeeshan; Trigun, S K; Joshi, Namrata (2024). Pharmaceutical characterization and exploration of Arkeshwara rasa in MDA-MB-231 cells. Journal of Ayurveda and integrative medicine, 15(1), p. 100823. Elsevier 10.1016/j.jaim.2023.100823
|
Text
1-s2.0-S0975947623001407-main.pdf - Published Version Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND). Download (3MB) | Preview |
BACKGROUND
The diverse specificity mode of cancer treatment targets and chemo resistance demands the necessity of drug entities which can address the devastating dynamicity of the disease.
OBJECTIVES
To check the anti-tumour potential of traditional medicine rich in polyherbal components and metal nanoparticle namely Arkeshwara rasa (AR).
MATERIAL METHODS
The AR was prepared in a modified version with reference from Rasaratna Samuchaya and characterized using sophisticated instrumental analysis including XRD, SEM-EDAX, TEM, TGA-DSC, and LC-MS and tested against the MDA-MB-231 cell line to screen cell viability and the cytotoxicity with MTT, SRB and the AO assay.
RESULTS
XRD pattern shows cubic tetrahedrite structure with Sb, Cu, S peaks and trace elements like Fe, Mg, etc. The particle size of AR ranges between 20 and 30 nm. The TGA points thermal decomposition at 210 °C and the metal sulphide peaks in DSC. LC-MS analysis reveals the components of the formulation more on the flavonoid portion. The IC50 value of MTT and SRB are 25.28 μg/mL and 31.7 μg/mL respectively. The AO colorimeter substantiated the cell viability and the apoptosis figures of the same cell line. The AR exhibits cytotoxicity and reaffirms the apoptosis fraction with SRB assay.
CONCLUSIONS
The Hesperidine, Neohesperidin, Rutin components in the phytochemical pool can synergize the anti-tumour potential with either influencing cellular pathways or decreasing chemo resistance to conventional treatment. AR need to be further experimented with reverse transcription, flow cytometry, western blotting, etc.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine |
UniBE Contributor: |
Cholke, Kaushavi Anil |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
0975-9476 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
04 Jan 2024 09:00 |
Last Modified: |
17 Mar 2024 00:15 |
Publisher DOI: |
10.1016/j.jaim.2023.100823 |
PubMed ID: |
38160612 |
Uncontrolled Keywords: |
Arkeshwara rasa Cancer Hesperidin IC(50) value In-vitro Traditional medicine |
BORIS DOI: |
10.48350/191072 |
URI: |
https://boris.unibe.ch/id/eprint/191072 |
Actions (login required)
 |
Edit item |